Du Plessis was in no mood to play the role of a sidekick as he waded into left-arm pacer Josh Little with a sequence of 4, 6, 4, 4 in the second over that produced a total of 20 runs.
The Titans introduced IPL debutant left-arm spinner Manav Suthar in the hope of containing Kohli at least, but the ploy was foiled spectacularly.
The RCB talisman showed his increasing comfort against spin, clobbering Suthar for two successive sixes.
Du Plessis reserved his punitive blade for Mohit, whom he carded around for four fours in the fifth over, as the South African reached fifty in just 18 balls before falling to Little.
RCB entered a phase of struggle from there as they lost last match centurion Will Jacks, Rajat Patidar, Glenn Maxwell and Cameron Green between the sixth and 10th overs for just 20 runs.
Little (4/45) and spinner Noor Ahmad (2/23) were the chief perpetrators as RCB slipped from 92 for 1 to 112 for five.
Ahmad soon ousted Kohli as RCB were 117 for six but Dinesh Karthik (21 not out, 12b) was cool under pressure as RCB notched their overall fourth and third win in a row of this season.
Before RCB batsmen joined the party, their bowlers came up with an immaculate effort on a supportive pitch to bowl GT to a modest 147.
There were a couple of good partnerships – 61 between David Miller and Shahrukh Khan and 44 by Rahul Tewatia and Rashid Khan – but the Titans lacked that one big innings or a stand that could have given them a firm grip on the match.
In fact, they seemed distinctly incapable of accelerating even in the Power Play as their top-order batters battled against RCB pacers, Mohammed Siraj (2/29) and Yash Dayal (2/21) who stuck to a wonderful length.
That GT managed to strike just two fours in Power Play offered ample evidence to their struggle and RCB bowlers’ accuracy.
The Gujarat side’s Power Play score of 23 for three was the lowest in the segment this season, and it was mainly due to Siraj’s domination upfront.
He consumed an out-of-form Wriddhiman Saha with a fine outswinger which the GT opener wafted to Dinesh Karthik behind the stumps.
Shubman Gill followed soon as his attempted swat to the on-side off Siraj took a leading edge and ended in the hands of Vysakh Vijayakumar at deep point.
Green fetched the third wicket for RCB in the Power Play when he jettisoned in-form B Sai Sudharsan, whose feeble pull close to the body was snaffled by Kohli at mid-off.
Miller (30, 20b) and Shahrukh (37, 24b) tried their best to drag GT out of the woods with a well-paced 61-run alliance off 37 balls for the fourth wicket.
Miller, who was fortunate to get dropped on 23 off Green by Karn Sharma, played archetypal power shots.
He punished leg-spinner Karn for a couple of sixes – a pull and a loft over extra cover – but fell to the same bowler when a miscued heave was grabbed by Maxwell at deep.
But a bigger misfortune was in store for the visitors as Shahrukh, who backed up a tad too far to Rahul Tewatia’s soft drop, could not beat a throw from Kohli to the non-striker’s end.
For the sixth-wicket, Tewatia (35, 21b) and Rashid (18, 14b) added 44 off 29 balls, but the latter’s desire to be innovative against Yash Dayal saw the ball crashing onto the stumps.
Tewatia, who punished Karn for 18 runs (4, 6, 4, 4) in the 16th over, departed soon.
